The effect of rotor velocity on sedimentation coefficients.

Variation of sedimentation coefficient with rotor velocity.

The sedimentation coefficients of three proteins, which appeared homogeneous in the ultracentrifuge, have been determined at different rotor velocities. Two of the proteins, ovalbumin and human gamma-globulin, showed sedimentation coefficients that were approx. 10% higher at rotor velocities below 20000rev./min. than at 56100rev./min. The third protein, haemocyanin of Jasus lalandii, could not ...

Sedimentation velocity, multi-speed method for analyzing polydisperse solutions.

A method is described for the sedimentation velocity analysis of solutions composed of macromolecular solutes of widely disparate size. In sedimentation velocity experiments, usually a single rotor speed is chosen for the entire run, and consequently, the range of observable sedimentation coefficients can be severely limited. This limitation can be removed if the speed is varied during the run,...

A model for sedimentation in inhomogeneous media. II. Compressibility of aqueous and organic solvents.

The effects of solvent compressibility on the sedimentation behavior of macromolecules as observed in analytical ultracentrifugation are examined. Expressions for the density and pressure distributions in the solution column are derived and combined with the finite element solution of the Lamm equation in inhomogeneous media to predict the macromolecular concentration distributions under differ...

The effect of feed ingredients on the settling velocity of feces in Tilapia (Oreochromis niloticus L.)

3D-Printing for Analytical Ultracentrifugation

Analytical ultracentrifugation (AUC) is a classical technique of physical biochemistry providing information on size, shape, and interactions of macromolecules from the analysis of their migration in centrifugal fields while free in solution. A key mechanical element in AUC is the centerpiece, a component of the sample cell assembly that is mounted between the optical windows to allow imaging a...